Anti-Proteasome 19S S5A/ASF 兔多克隆抗体-细胞器标志物-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-Proteasome 19S S5A/ASF 兔多克隆抗体

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货号： K1341385-100

预测分子量： 41 kDa

观测分子量： 55 kDa

别名： AF,AF 1,Antisecretory factor 1,ASF,MCB1,PSMD4,pUB R5,Rpn10,S5A,26S proteasome regulatory subunit RPN10,26S proteasome regulatory subunit S5A,Multiubiquitin chain-binding protein

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	26S蛋白酶体非ATP酶调节亚基4
别名	AF,AF 1,Antisecretory factor 1,ASF,MCB1,PSMD4,pUB R5,Rpn10,S5A,26S proteasome regulatory subunit RPN10,26S proteasome regulatory subunit S5A,Multiubiquitin chain-binding protein
Uniprot ID	O35226
免疫原	KLH偶联的小鼠Proteasome 19S S5A/ASF合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	41 kDa / 55 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:睾丸

背景

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. This antibody is specific to PSMD4.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Proteasome 19S S5A/ASF蛋白(货号 K1341385).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.