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Anti-MED28/EG1 兔多克隆抗体
¥1900
加入对比
货号: K1342543-100
预测分子量: 20 kDa
观测分子量: 24 kDa
别名: 1500003D12Rik,EG1,Endothelial derived protein 1,magicin,MED28,mediator complex subunit 28,Tumor angiogenesis marker EG 1
应用 | 种属
WB M,R
规格:
产品信息
蛋白质全称 中介复合体亚基28
别名 1500003D12Rik,EG1,Endothelial derived protein 1,magicin,MED28,mediator complex subunit 28,Tumor angiogenesis marker EG 1
Uniprot ID Q920D3
免疫原 小鼠MED28/EG1重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 20 kDa / 24 kDa
应用
WB 免疫印迹
物种:小鼠,大鼠
稀释比:1:700-1:1400
阳性样品:脑
背景
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways (By similarity).
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测MED28/EG1蛋白(货号 K1342543).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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