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Anti-EMC4 兔多克隆抗体
¥1900
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货号: K1342555-100
预测分子量: 20 kDa
观测分子量: 17 kDa
别名: EMC4,ER membrane protein complex subunit 4,PIG17,TMEM85,transmembrane protein 85
应用 | 种属
WB H,M
规格:
产品信息
蛋白质全称 ER膜蛋白复合体亚基4
别名 EMC4,ER membrane protein complex subunit 4,PIG17,TMEM85,transmembrane protein 85
Uniprot ID Q9CZX9
免疫原 小鼠EMC4重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 20 kDa / 17 kDa
应用
WB 免疫印迹
物种:人,小鼠
稀释比:1:300-1:600
阳性样品:肝, 脑
背景
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测EMC4蛋白(货号 K1342555).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 300稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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