Anti-UCHL3 兔多克隆抗体-泛素化相关-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-UCHL3 兔多克隆抗体

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货号： K1345804-100

预测分子量： 26 kDa

观测分子量： 26 kDa

别名： Ubiquitin carboxyl terminal hydrolase isozyme L3,Ubiquitin thioesterase L3,UCHL3,UCH-L3

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	泛素羧基末端水解酶同工酶L3
别名	Ubiquitin carboxyl terminal hydrolase isozyme L3,Ubiquitin thioesterase L3,UCHL3,UCH-L3
Uniprot ID	Q9JKB1
免疫原	KLH偶联的小鼠UCHL3合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	26 kDa / 26 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:1000-1:2000

阳性样品:睾丸

背景

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3'', and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测UCHL3蛋白(货号 K1345804).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.