Anti-Gemin6/SIP2 兔多克隆抗体-RNA加工-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-Gemin6/SIP2 兔多克隆抗体

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￥1900

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货号： K1346353-100

预测分子量： 19 kDa

观测分子量： 19 kDa

别名： Gem associated protein 6,Gemin 6,GEMIN6,SIP2

应用 | 种属

WB M

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	GEM关联蛋白6
别名	Gem associated protein 6,Gemin 6,GEMIN6,SIP2
Uniprot ID	Q8WXD5
免疫原	人Gemin6重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	19 kDa / 19 kDa

应用

WB 免疫印迹

物种:小鼠

稀释比:1:500-1:1000

阳性样品:胰腺

背景

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Gemin6/SIP2蛋白(货号 K1346353).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.