Anti-RALBP1 兔多克隆抗体-其它-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-RALBP1 兔多克隆抗体

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货号： K1346794-100

亚细胞定位：细胞膜, 细胞质, 细胞骨架

别名： 76 kDa Ral interacting protein,DNP SG ATPase,Ral interacting protein 1,ralA binding protein 1,RALBP1,RIP1

应用 | 种属

IHC/IF H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	RalA结合蛋白1
别名	76 kDa Ral interacting protein,DNP SG ATPase,Ral interacting protein 1,ralA binding protein 1,RALBP1,RIP1
Uniprot ID	Q62172
免疫原	KLH偶联的小鼠RALBP1合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞膜, 细胞质, 细胞骨架

应用

IHC/IF 免疫组织化学/免疫荧光

物种:人,小鼠,大鼠

稀释比:1:150-1:600

阳性样品:肾, 骨骼肌

背景

Multifunctional protein that functions as a downstream effector of RALA and RALB. As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity. As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis. During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle. During mitosis, also controls mitochondrial fission as an effector of RALA. Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L. Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin contributing to cell multidrug resistance.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测RALBP1蛋白(货号 K1346794).
样品: 人肾, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 98℃, 20分钟.
—抗: 1: 400稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.

IHC检测RALBP1蛋白(货号 K1346794).
样品: 小鼠骨骼肌, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 98℃, 20分钟.
—抗: 1: 400稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.

IHC检测RALBP1蛋白(货号 K1346794).
样品: 大鼠骨骼肌, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 98℃, 20分钟.
—抗: 1: 400稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.