Recombinant Anti-8-hydroxydeoxyguanosine antibody (小鼠单克隆抗体)-其它,重组抗体-科普欣生物 Cookbio 抗体|仪器|耗材|试剂-武汉科普欣生物科技有限公司

Recombinant Anti-8-hydroxydeoxyguanosine antibody (小鼠单克隆抗体)

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货号： K5450345-100

亚细胞定位：细胞核

别名： 8-OHdG,8-hydroxydeoxyguanosine,8-hydroxy-2'-deoxyguanosine,8-oxo-2'-deoxyguanosine

应用 | 种属

IHC/IF Species independent

规格：

100 μL 50 μL

产品描述
应用
背景
储存条件
图像

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产品信息

别名	8-OHdG,8-hydroxydeoxyguanosine,8-hydroxy-2'-deoxyguanosine,8-oxo-2'-deoxyguanosine
抗体亚型	IgG1
克隆号	SB482
纯化方式	亲和纯化
亚细胞定位	细胞核

应用

IHC/IF 免疫组织化学/免疫荧光

物种:Species independent

稀释比:1:200-1:800

阳性样品:非酒精性脂肪性肝病, 肿瘤

背景

8-hydroxydeoxyguanosine (8-OHdG) is a derivative of deoxyguanosine nucleotide with a hydroxyl group attached to the C8 carbon. This modification alters the base of the DNA molecule, thereby affecting its stability and function. It is an oxidative adduct produced when reactive oxygen species such as hydroxyl radicals and singlet oxygen attack the 8th carbon atom of the guanine base in the DNA molecule. This oxidative reaction is one of the main forms of DNA damage and is closely related to the aging of the body and the occurrence of diseases such as cancer, cardiovascular diseases, diabetes, and neurodegenerative diseases. As a biomarker of DNA oxidative damage caused by endogenous and exogenous factors, 8-OHdG is a very promising indicator.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测8-hydroxydeoxyguanosine蛋白(货号 K5450345).
样品: 肿瘤, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 100℃, 25分钟.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗小鼠), 即用型(货号KB3903), 室温孵育20分钟.

IHC检测8-hydroxydeoxyguanosine蛋白(货号 K5450345).
样品: 小鼠非酒精性脂肪性肝病, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 100℃, 25分钟.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗小鼠), 即用型(货号KB3903), 室温孵育20分钟.

IHC检测8-hydroxydeoxyguanosine蛋白(货号 K5450345).
样品: 大鼠非酒精性脂肪性肝病, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 100℃, 25分钟.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗小鼠), 即用型(货号KB3903), 室温孵育20分钟.