Recombinant Anti-Beclin 1 antibody (兔单克隆抗体)-自噬,重组抗体-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Recombinant Anti-Beclin 1 antibody (兔单克隆抗体)

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￥1900

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货号： K5467223-100

预测分子量： 52 kDa

观测分子量： 60 kDa

别名： Coiled-coil myosin-like BCL2-interacting protein,Protein GT197,BECN1,GT197,APG6,VPS30,Protein GT197,beclin1,beclin-1

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	Beclin-1自噬相关蛋白
别名	Coiled-coil myosin-like BCL2-interacting protein,Protein GT197,BECN1,GT197,APG6,VPS30,Protein GT197,beclin1,beclin-1
Uniprot ID	O88597
免疫原	小鼠Beclin 1重组蛋白
抗体亚型	IgG
克隆号	SB315
纯化方式	亲和纯化
预测分子量./观测分子量.	52 kDa / 60 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:500-1:2000

阳性样品:脑

背景

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Beclin 1蛋白(货号 K5467223).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.