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Recombinant Anti-Ube2N/Ubc13 antibody (兔单克隆抗体)
¥1900
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货号: K547958-100
预测分子量: 17 kDa
观测分子量: 17 kDa
别名: Ube2n,Bendless like ubiquitin conjugating enzyme,BLU,UBC 13,Ube2n,Ubiquitin carrier protein N,Ubiquitin conjugating enzyme E2N
应用 | 种属
WB H,M,R
规格:
产品信息
蛋白质全称 泛素结合酶E2N
别名 Ube2n,Bendless like ubiquitin conjugating enzyme,BLU,UBC 13,Ube2n,Ubiquitin carrier protein N,Ubiquitin conjugating enzyme E2N
Uniprot ID P61089
免疫原 小鼠UBE2N重组蛋白
抗体亚型 IgG, κ
克隆号 SB336
纯化方式 亲和纯化
预测分子量./观测分子量. 17 kDa / 17 kDa
应用
WB 免疫印迹
物种:人,小鼠,大鼠
稀释比:1:500-1:1000
阳性样品:293细胞, 脑
背景
UBC13 is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. Existing as a heterodimer with UBE2V2, UBC13 catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. Additionally, UBC13 mediates the transcription of several target genes and is thought to play a role in cell cycle progression, cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测Ube2N/Ubc13蛋白(货号 K547958).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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