Recombinant Anti-Melanoma gp100 antibody (兔单克隆抗体)-肿瘤标志物,重组抗体-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Recombinant Anti-Melanoma gp100 antibody (兔单克隆抗体)

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货号： K5450450-100

亚细胞定位：黑色素体, 细胞质

别名： 95 kDa melanocyte specific secreted glycoprotein,95 kDa melanocyte-specific secreted glycoprotein,D12S53E,gp100,M-beta,ME20,ME20 M/ME20 S,ME20-M,ME20-S,ME20M,ME20M/ME20S,ME20S,Melanocyte lineage specific antigen GP100,Melanocyte protein mel 17,Melanocyte protein Pmel 17,Melanocyte protein Pmel 17 precursor,Melanocytes lineage-specific antigen GP100,Melanoma associated ME20 antigen,Melanoma gp100,Melanoma-associated ME20 antigen,Melanosomal matrix protein 17,Melanosomal matrix protein17,P1,p100,p26,PMEL 17,PMEL,Premelanosome protein,Secreted melanoma-associated ME20 antigen,SI,SIL,D12S53E,PMEL17,SILV,PMEL

应用 | 种属

IHC/IF H

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	黑素细胞蛋白PMEL
别名	95 kDa melanocyte specific secreted glycoprotein,95 kDa melanocyte-specific secreted glycoprotein,D12S53E,gp100,M-beta,ME20,ME20 M/ME20 S,ME20-M,ME20-S,ME20M,ME20M/ME20S,ME20S,Melanocyte lineage specific antigen GP100,Melanocyte protein mel 17,Melanocyte protein Pmel 17,Melanocyte protein Pmel 17 precursor,Melanocytes lineage-specific antigen GP100,Melanoma associated ME20 antigen,Melanoma gp100,Melanoma-associated ME20 antigen,Melanosomal matrix protein 17,Melanosomal matrix protein17,P1,p100,p26,PMEL 17,PMEL,Premelanosome protein,Secreted melanoma-associated ME20 antigen,SI,SIL,D12S53E,PMEL17,SILV,PMEL
Uniprot ID	P40967
免疫原	人Melanoma gp100重组蛋白
抗体亚型	IgG
克隆号	SB575
纯化方式	亲和纯化
亚细胞定位	黑色素体, 细胞质

应用

IHC/IF 免疫组织化学/免疫荧光

物种:人

稀释比:1:100-1:250

阳性样品:黑色素瘤

背景

Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测Melanoma gp100蛋白(货号 K5450450).
样品: 人黑色素瘤, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (货号KSG1203), 水浴100℃, 25分钟.
—抗: 1: 200稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.