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Anti-Glutamine Synthetase 兔多克隆抗体
¥1900
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货号: K1333651-100
预测分子量: 42 kDa
观测分子量: 42 kDa
别名: cell proliferation-inducing protein 59,GLNA,GLNS,GLUL,Glutamate ammonia ligase,Glutamate decarboxylase,Glutamine synthetase,GS,PIG43,PIG59,Proliferation inducing protein 43,Palmitoyltransferase GLUL
应用 | 种属
WB M,R
规格:
产品信息
蛋白质全称 谷氨酰胺合成酶
别名 cell proliferation-inducing protein 59,GLNA,GLNS,GLUL,Glutamate ammonia ligase,Glutamate decarboxylase,Glutamine synthetase,GS,PIG43,PIG59,Proliferation inducing protein 43,Palmitoyltransferase GLUL
Uniprot ID P15105
免疫原 人Glutamine Synthetase重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 42 kDa / 42 kDa
应用
WB 免疫印迹
物种:小鼠,大鼠
稀释比:1:500-1:1000
阳性样品:脑
背景
Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 42 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测Glutamine Synthetase蛋白(货号 K1333651).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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