Anti-LC3A/B 兔多克隆抗体-自噬-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-LC3A/B 兔多克隆抗体

咨询

￥1900

加入对比

货号： K133372-100

预测分子量： 14/16 kDa

观测分子量： 14/16 kDa

亚细胞定位：胞质, 自噬体膜

别名： LC3,LC3A,LC3A/B,LC3B,MAP1A/MAP1B LC3 A,MAP1A/MAP1B light chain 3 A,MAP1ALC3,MAP1BLC3,MAP1LC3A,MAP1LC3A/B,MAP1LC3B

应用 | 种属

WB H,M,R

ICC/IF H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

在线预览下载说明书

产品信息

蛋白质全称	微管关联蛋白1A/1B轻链3
别名	LC3,LC3A,LC3A/B,LC3B,MAP1A/MAP1B LC3 A,MAP1A/MAP1B light chain 3 A,MAP1ALC3,MAP1BLC3,MAP1LC3A,MAP1LC3A/B,MAP1LC3B
Uniprot ID	Q9CQV6
免疫原	KLH偶联的小鼠LC3B合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	胞质, 自噬体膜
预测分子量./观测分子量.	14/16 kDa / 14/16 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:1000-1:2000

阳性样品:海马区

ICC/IF 免疫细胞化学/免疫荧光

物种:人,小鼠,大鼠

稀释比:1:300-1:600

阳性样品:—

背景

LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测LC3A/B蛋白(货号 K133372).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.