Anti-Nesprin3 兔多克隆抗体-细胞连接-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-Nesprin3 兔多克隆抗体

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货号： K1340326-100

预测分子量： 112 kDa

观测分子量： 112 kDa

别名： KASH domain-containing protein 3,KASH3,Nuclear envelope spectrin repeat protein 3,Syne3,C14orf49,Spectrin Repeat Containing,Nuclear Envelope Family Member 3,Nesp3,Chromosome 14 open reading frame 49,NET53

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	Nesprin-3
别名	KASH domain-containing protein 3,KASH3,Nuclear envelope spectrin repeat protein 3,Syne3,C14orf49,Spectrin Repeat Containing,Nuclear Envelope Family Member 3,Nesp3,Chromosome 14 open reading frame 49,NET53
Uniprot ID	Q4FZC9
免疫原	小鼠Nesprin3重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	112 kDa / 112 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:300-1:800

阳性样品:睾丸, 脾

背景

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Nesprin3蛋白(货号 K1340326).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.