Recombinant Anti-PODXL antibody (兔单克隆抗体)-细胞连接,重组抗体-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Recombinant Anti-PODXL antibody (兔单克隆抗体)

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￥1900

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货号： K5450060-100

亚细胞定位：细胞膜

别名： GCTM 2 antigen,Gp200,PC,PCLP,PCLP-1,PCLP1,Podocalyxin,podocalyxin like,Podocalyxin like protein 1,PODXL

应用 | 种属

IHC/IF H

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	足糖萼蛋白
别名	GCTM 2 antigen,Gp200,PC,PCLP,PCLP-1,PCLP1,Podocalyxin,podocalyxin like,Podocalyxin like protein 1,PODXL
Uniprot ID	O00592
免疫原	人PODXL重组蛋白
抗体亚型	IgG, κ
克隆号	SB224
纯化方式	亲和纯化
亚细胞定位	细胞膜

应用

IHC/IF 免疫组织化学/免疫荧光

物种:人

稀释比:1:500-1:1000

阳性样品:食管癌, 肾

背景

Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测PODXL蛋白(货号 K5450060).
样品: 人食管癌, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (KSG1201), 高压锅均匀喷气计时2分钟.
—抗: 1: 800稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.

IHC检测PODXL蛋白(货号 K5450060).
样品: 人肾, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (KSG1201), 高压锅均匀喷气计时2分钟.
—抗: 1: 800稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.