Anti-TATA binding protein TBP 兔多克隆抗体-内参抗体,转录因子-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-TATA binding protein TBP 兔多克隆抗体

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￥1900

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货号： K1341430-100

预测分子量： 35 kDa

观测分子量： 35 kDa

别名： GTF2D,GTF2D1,SCA17,TATA-binding factor,TATA-box factor,TATA sequence binding protein,TBP,TF2D,TFIID

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	TATA框结合蛋白
别名	GTF2D,GTF2D1,SCA17,TATA-binding factor,TATA-box factor,TATA sequence binding protein,TBP,TF2D,TFIID
Uniprot ID	P29037
免疫原	KLH偶联的小鼠TBP合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	35 kDa / 35 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:200-1:500

阳性样品:睾丸

背景

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测TATA binding protein TBP蛋白(货号 K1341430).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.