Recombinant Anti-HA Tag antibody (小鼠单克隆抗体)-标签抗体,重组抗体-科普欣生物 Cookbio 抗体|仪器|耗材|试剂-武汉科普欣生物科技有限公司

Recombinant Anti-HA Tag antibody (小鼠单克隆抗体)

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￥980

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货号： K5453756-100

别名： HA epitope tag,HA1,HA2,hemagglutinin,Hemagglutinin HA1 chain,Hemagglutinin HA2 chain

应用 | 种属

WB Species independent

ICC/IF Species independent

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	HA标签
别名	HA epitope tag,HA1,HA2,hemagglutinin,Hemagglutinin HA1 chain,Hemagglutinin HA2 chain
免疫原	KLH偶联的YPYDVPDYA合成肽
抗体亚型	IgG1, κ
克隆号	SB12
纯化方式	亲和纯化

应用

WB 免疫印迹

物种:Species independent

稀释比:1:1000-1:2000

阳性样品:—

ICC/IF 免疫细胞化学/免疫荧光

物种:Species independent

稀释比:1:1000-1:2000

阳性样品:293细胞

背景

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

ICC检测HA Tag蛋白(货号 K5453756)(红色）.
样品: 293细胞, 4%多聚甲醛 (货号KSG1101) 固定20分钟.
封闭: 3% BSA（货号KSGC305010）的PBS溶液, 室温孵育30分钟.
—抗: 1: 1500稀释, 4℃ 孵育过夜.
二抗: Cy3标记山羊抗小鼠IgG (H+L) (货号KB63903), 1: 300稀释, 室温孵育1小时.