Anti-Mob1A 兔多克隆抗体-凋亡-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-Mob1A 兔多克隆抗体

咨询

￥1900

加入对比

货号： K1342942-100

预测分子量： 25 kDa

观测分子量： 25 kDa

别名： MATS2,Mob1 homolog 1A,Mob1A,Mob1B,MOB4A,MOBKL1A,Protein Mob4A

应用 | 种属

WB H,M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

在线预览下载说明书

产品信息

蛋白质全称	MOB激酶激活剂1A
别名	MATS2,Mob1 homolog 1A,Mob1A,Mob1B,MOB4A,MOBKL1A,Protein Mob4A
Uniprot ID	Q921Y0
免疫原	KLH偶联的小鼠Mob1A合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	25 kDa / 25 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:400-1:800

阳性样品:脊髓

背景

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38 (By similarity).

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Mob1A蛋白(货号 K1342942).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.