Anti-ACAA2 兔多克隆抗体-脂代谢-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

热门搜索： CD3 Actin GFP

Anti-ACAA2 兔多克隆抗体

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￥1900

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货号： K1344160-100

预测分子量： 42 kDa

观测分子量： 42 kDa

别名： ACAA2,Acetyl CoA acyltransferase,Beta ketothiolase,DSAEC,T1

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	线粒体3-酮酰基-CoA硫解酶
别名	ACAA2,Acetyl CoA acyltransferase,Beta ketothiolase,DSAEC,T1
Uniprot ID	Q8BWT1
免疫原	KLH偶联的小鼠ACAA2合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	42 kDa / 42 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:1500-1:3000

阳性样品:脑

背景

In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测ACAA2蛋白(货号 K1344160).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.