Recombinant Anti-Met (c-Met) antibody (兔单克隆抗体)-原癌/抑癌基因蛋白,生长因子及受体,重组抗体-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Recombinant Anti-Met (c-Met) antibody (兔单克隆抗体)

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￥1900

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货号： K5450516-100

预测分子量： 156 kDa

观测分子量： 170 kDa

亚细胞定位：膜, 分泌型

别名： MET,Scatter factor receptor (SF receptor),Tyrosine-protein kinase Met

应用 | 种属

WB H

IHC/IF H

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	肝细胞生长因子受体
别名	MET,Scatter factor receptor (SF receptor),Tyrosine-protein kinase Met
Uniprot ID	P08581
免疫原	KLH偶联的人HGFR/c-MET合成肽
抗体亚型	IgG
克隆号	SB608
纯化方式	亲和纯化
亚细胞定位	膜, 分泌型
预测分子量./观测分子量.	156 kDa / 170 kDa

应用

WB 免疫印迹

物种:人

稀释比:1:500-1:2000

阳性样品:—

IHC/IF 免疫组织化学/免疫荧光

物种:人

稀释比:1:1000-1:3000

阳性样品:肾, 卵巢癌

背景

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测Met (c-Met)蛋白(货号 K5450516).
样品: 人卵巢癌, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (货号KSG1203), 水浴100℃, 25分钟.
—抗: 1: 2000稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.

IF检测Met (c-Met)蛋白(货号 K5450516)(红色）.
样品: 人肾, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (货号KSG1203), 水浴100℃, 25分钟.
封闭: 3% BSA（货号KSGC305010）的PBS溶液, 室温孵育30分钟.
—抗: 1: 2000稀释, 4℃ 孵育过夜.
二抗: Cy3标记山羊抗兔IgG (H+L) (货号KB63909), 1: 300稀释, 室温孵育1小时.