Anti-SOX9 兔多克隆抗体-转录因子-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-SOX9 兔多克隆抗体

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￥1900

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货号： K133840-100

预测分子量： 56 kDa

观测分子量： 56 kDa

别名： CMD1,CMPD1,SOX9,SRA1,Transcription factor SOX 9,SRXX2,SRXY10,SRY-box 9

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	转录因子SOX-9
别名	CMD1,CMPD1,SOX9,SRA1,Transcription factor SOX 9,SRXX2,SRXY10,SRY-box 9
Uniprot ID	Q04887
免疫原	KLH偶联的小鼠SOX9合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	56 kDa / 56 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:脑, 肝

背景

Transcription factor that plays a key role in chondrocytes differentiation and skeletal development. Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6. Also binds to some promoter regions. Plays a central role in successive steps of chondrocyte differentiation. Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes. Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测SOX9蛋白(货号 K133840).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 500稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.