Anti-TDP43 兔多克隆抗体-转录因子-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-TDP43 兔多克隆抗体

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货号： K1336480-100

亚细胞定位：细胞核, 细胞质

别名： TDP-43,Tardbp,ALS10,Tdp43,TAR DNA binding protein

应用 | 种属

IHC/IF M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	TAR DNA结合蛋白43
别名	TDP-43,Tardbp,ALS10,Tdp43,TAR DNA binding protein
Uniprot ID	Q921F2
免疫原	KLH偶联的小鼠TDP43合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞核, 细胞质

应用

IHC/IF 免疫组织化学/免疫荧光

物种:小鼠,大鼠

稀释比:1:200-1:400

阳性样品:脑, 睾丸

背景

RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening. In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测TDP43蛋白(货号 K1336480).
样品: 小鼠脑, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 98℃, 20分钟.
—抗: 1: 300稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.

IHC检测TDP43蛋白(货号 K1336480).
样品: 大鼠睾丸, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (KSG1203), 98℃, 20分钟.
—抗: 1: 300稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号KB3906), 室温孵育20分钟.