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Anti-TRIP 兔多克隆抗体
¥1900
加入对比
货号: K1343878-100
预测分子量: 53 kDa
观测分子量: 55 kDa
别名: RING finger protein 206,RNF206,TRAF interacting protein,TRAIP,TRIP
应用 | 种属
WB H,M,R
规格:
产品信息
蛋白质全称 E3泛素连接酶TRAIP
别名 RING finger protein 206,RNF206,TRAF interacting protein,TRAIP,TRIP
Uniprot ID Q8VIG6
免疫原 KLH偶联的小鼠TRIP合成肽
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 53 kDa / 55 kDa
应用
WB 免疫印迹
物种:人,小鼠,大鼠
稀释比:1:500-1:1000
阳性样品:肝
背景
E3 ubiquitin ligase required to protect genome stability in response to replication stress . Acts as a key regulator of interstrand cross-link repair, which takes place when both strands of duplex DNA are covalently tethered together, thereby blocking replication and transcription . Controls the choice between the two pathways of replication-coupled interstrand-cross-link repair by mediating ubiquitination of MCM7 subunit of the CMG helicase complex . Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3 . If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase, enabling the Fanconi anemia DNA repair pathway .
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测TRIP蛋白(货号 K1343878).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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