Anti-Retinoic Acid Receptor alpha 兔多克隆抗体-激素及受体-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-Retinoic Acid Receptor alpha 兔多克隆抗体

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￥1900

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货号： K1346464-100

预测分子量： 50 kDa

观测分子量： 60 kDa

别名： NR1B1,RAR,RAR alpha,RARA,Retinoic acid receptor alpha,retinoic acid receptor,alpha

应用 | 种属

WB M,R

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	视黄酸受体α
别名	NR1B1,RAR,RAR alpha,RARA,Retinoic acid receptor alpha,retinoic acid receptor,alpha
Uniprot ID	P11416
免疫原	KLH偶联的小鼠RARa合成肽
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	50 kDa / 60 kDa

应用

WB 免疫印迹

物种:小鼠,大鼠

稀释比:1:300-1:500

阳性样品:睾丸

背景

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression . On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. Formation of heterocomplex with histone deacetylases might lead to inhibition of RARE DNA element binding and to transcriptional repression.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Retinoic Acid Receptor alpha蛋白(货号 K1346464).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 300稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.