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Anti-CD320 兔多克隆抗体
¥1900
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货号: K1342390-100
预测分子量: 28 kDa
观测分子量: 27 kDa
别名: 8D6,8D6 antigen,8D6A,CD320,CD320 antigen,CD320 molecule,FDC signaling molecule 8D6,FDC SM 8D6,TCblR,Transcobalamin receptor
应用 | 种属
WB M,R
规格:
产品信息
蛋白质全称 CD320抗原
别名 8D6,8D6 antigen,8D6A,CD320,CD320 antigen,CD320 molecule,FDC signaling molecule 8D6,FDC SM 8D6,TCblR,Transcobalamin receptor
Uniprot ID Q9Z1P5
免疫原 小鼠CD320重组蛋白
抗体亚型 IgG
纯化方式 亲和纯化
预测分子量./观测分子量. 28 kDa / 27 kDa
应用
WB 免疫印迹
物种:小鼠,大鼠
稀释比:1:500-1:1000
阳性样品:肺
背景
CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa. This antibody raised against 84-280aa of human CD320 protein detects bands at 58 kDa and 60-70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation .
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
WB检测CD320蛋白(货号 K1342390).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.

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