Recombinant Anti-Glypican 3 antibody (小鼠单克隆抗体)-细胞骨架,重组抗体-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Recombinant Anti-Glypican 3 antibody (小鼠单克隆抗体)

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￥1900

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货号： K5466587-100

亚细胞定位：细胞膜

别名： DGSX,Glypican 3,GPC3,GTR2 2,Intestinal protein OCI 5,MXR7,OCI 5,OCI5,SDYS,SGB,SGBS,SGBS1

应用 | 种属

IHC/IF H

规格：

100 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	磷脂酰肌醇蛋白聚糖3
别名	DGSX,Glypican 3,GPC3,GTR2 2,Intestinal protein OCI 5,MXR7,OCI 5,OCI5,SDYS,SGB,SGBS,SGBS1
Uniprot ID	P51654
免疫原	人Glypican 3重组蛋白
抗体亚型	IgG1, κ
克隆号	SB579
纯化方式	亲和纯化
亚细胞定位	细胞膜

应用

IHC/IF 免疫组织化学/免疫荧光

物种:人

稀释比:1:500-1:1000

阳性样品:肝癌

背景

Cell surface proteoglycan that bears heparan sulfate . Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands . Positively regulates the non-canonical Wnt signaling pathway Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

IHC检测Glypican 3蛋白(货号 K5466587).
样品: 人肝癌, 4%多聚甲醛 (货号KSG1101) 固定12-24小时.
抗原修复: Tris-EDTA抗原修复液(pH 9.0) (货号KSG1203), 水浴100℃, 25分钟.
—抗: 1: 800稀释, 4℃ 孵育过夜.
二抗: S-vision免疫组化多聚二抗(山羊抗小鼠), 即用型(货号KB3903), 室温孵育20分钟.